BCR-ABL RNA quantitation is available from MolecularMD for monitoring response to imatinib (Gleevec ®) treatment, bone marrow transplantation, or related kinase inhibitor therapies. RNA extracted from blood or bone marrow aspirate is converted to cDNA, which is then subjected to real-time quantitative PCR (RQ-PCR). To normalize between patient samples, the amount of ABL transcript is measured simultaneously, with the results presented as a BCR-ABL/ABL ratio. This ratio is tracked over time on serially-submitted patient samples
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Test Specifications
| BCR-ABL RNA Quantitation |
| Sensitivity |
One tumor cell in 1x106 normal cells |
| Specificity |
Major breakpoint BCR b2a2, b3a2 |
| Internal Control |
RNA from cell lines that are negative and positive for BCR-ABL expression |
| Interassay Variability |
Above MMR: less than 2-fold
Below MMR: less than 3-fold |
| Intraassay Variability |
CV 5% |
| Specimen Requirement |
10cc of whole blood in an EDTA tube or 5cc of whole blood in two PAXgene tubes |
| Result Reporting |
BCR-ABL/ABL ratio, log change from previous test, log reduction from median baseline and overall sensitivity value |
| Turnaround Time |
4-5 business days. MolecularMD is open on Saturdays |
Please see shipping guidelines document for shipping instructions.
Please see BCR-ABL Quantitation Spec Sheet for sample test results.
Why Choose MolecularMD for your BCR-ABL RNA Quantitation Services?
High Sensitivity: The major molecular responses that are indicative of patient responsiveness to imatinib or related therapeutics tend to drive BCR-ABL transcript levels to the lower limits of detection for many assays. A more sensitive assay will therefore provide more accurate results at these lower levels.
Reliability: Patient results are compared from one testing date to the next to ensure that BCR-ABL levels remain low. To have confidence in these levels requires the most reliable assay possible.
Fast Turnaround Time: Knowing as soon as possible if a patient is developing a resistance to their therapy can be of tremendous value. This requires a very quick turnaround time. MolecularMD is open on Saturdays for receiving and processing samples. |
Example of Patient Disease Response Reporting
Examples of RQ-PCR Sensitivity and Precision 
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